br Acknowledgments br Authors would like to thank
Authors would like to thank M.S. M. Diaz-Duarte for the colla-boration in the synthesis of anionic nanogels. This investigation was supported by the National Council of Science and Technology of Mexico (CONACYT) through the following grants: Cátedras CONACYT No707, CB2012-178709, and INFR-2016-269007. We thank S. Pérez-Sicairos and M. Salazar-Gastelum for ICP measurements, and V. Miranda-Soto for NMR measurements. The authors are grateful to three referees for their valuable comments in this Paclitaxel work.
Appendix A. Supplementary data
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Caudatin potentiates the anti-tumor eﬀects of TRAIL against human breast T cancer by upregulating DR5
Fei Hong-ronga,1, Yuan Chuangb,1, Wang Gui-linga, Zhao Yingb, Li Zhao-juna,b, Du Xinb, Wang Feng-Zeb,
a School of Pharmacy, Shandong First Medical University & Shandong Academy of Medical Sciences, Taian, 271016, PR China
b School of Life Sciences, Shandong First Medical University & Shandong Academy of Medical Sciences, Taian, 271016, PR China
Keywords: Background: The ability of tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) to preferentially
Caudatin induce apoptosis in transformed cells while sparing most normal cells is well established. However, the intrinsic
TRAIL and acquired resistance of tumors to TRAIL-induced apoptosis limits its therapeutic applicability.
DR5 Purpose: We investigated the eﬀect of caudatin, a species of C-21 steroidal glycosides isolated from the roots of
Cynanchum auriculatum, on TRAIL-induced apoptosis in human breast cancer cells.
Methods: Cell growth inhibition was evaluated by the CCK-8 assay. The cell cycle distribution was assessed by
propidium iodide flow cytometry. Apoptosis was determined by TUNEL staining. Protein expression was de-